TUBA1B anticorps

N° du produit ABIN238516

Cet anticorps Souris Monoclonal détecte spécifiquement TUBA1B dans WB. Il présente une réactivité envers Humain, Souris et Porc.

Aperçu rapide pour TUBA1B anticorps (ABIN238516)

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivité: Humain, Souris, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBA1B est non-conjugé

Application: Western Blotting (WB)

Clone: TU-02

Détail du produit anti-TUBA1B anticorps

Specificité: The antibody TU-02 recognizes an epitope on N-terminal structural domain of alpha-tubulin in various species.

Attributs du produit: Synonyms: Microtubule Marker, Loading Control, alpha-Tubulin, tubulin alpha

Purification: Precipitation methods.

Immunogène: microtubule proteins from porcine brain

Isotype: IgM

Information d'application

Indications d'application: Western Blotting (Reducing conditions): 1 μg/mLPositive control: porcine brain lysate. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur TUBA1B

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Autre désignation: alpha Tubulin / TUBA1B

Sujet: The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

ID gène: 10376

Pathways: Dynamique des Microtubules, M Phase